Journal of Rural Development and Agriculture (2016) 1(1): 39-48
39
Isolation and identification of Agrobacterium tumefaciens from
the galls of peach tree
Nizar Ali
1
, Akbar Zada
1
, Murad Ali
1
and Zahid Hussain
1
*
Key Message Agrobacterium tumefaciens attacks on peach plant and it causes crown gall disease. The
present study was conducted to isolate and identify Agrobacterium tumefaciens from crown gall samples of
peach plants.
ABSTRACT Peach (Prunus perisca) is a very important fruit and it is attractive all over the world due to its
delicious aroma and flavor. Peach is susceptible to various types of pathogens that result in decline of its fruit
production. An important pathogen, Agrobacterium tumefaciens attacks on peach plant and it causes crown
gall disease. The present study was conducted to identify the peach plants infected with Agrobacterium
tumefaciens and isolate as well as identify the bacterium from the crown gall samples. These samples were
collected from different locations including district Swat and Shangla. The bacterium was isolated from the
samples using MacConkey selection media that is used specifically for identification of Agrobacterium
tumefaciens. Two different biochemical tests i.e. Gram staining and KOH test were performed to confirm that
the isolated bacterium is gram negative. The biochemical tests reveal that all the bacterial isolates are gram
negative. Furthermore, two